Reporter

Part:BBa_K2005002:Design

Designed by: Jarrod Shilts   Group: iGEM16_Vanderbilt   (2016-10-18)


LacZ-alpha, UV-maximized


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To generate this artificial DNA sequence, we employed our custom-made algorithm for modifying gene sequences to introduce mutagenic sites. These sites were identified based on prior research on radiological damage, which identified dipyrimidine motifs as particularly vulnerable. These sites were identified based on prior research on DNA irradiation, where dipyrimidine motifs as particularly vulnerable. At these motifs, synonymous codon substitutions were made to make replacements for ones with higher mutation risks. In addition, our algorithm used heuristics to factor in the effect different codons have on gene expression to ensure that the gene not only produced the same protein but produced it at similar levels. Overall we could increase the predicted number of UV-mutation sites by 63%


Source

Synthetic variant of Bba_I732006

References